Anther Culture of Chinese Radish(Raphanus sativus L. var. Longinnatus Bailey):Response of Different Genotypes to the Embryogenesis and the Traits of Regenerated Plantlets
[Objective] The aims were to ① conduct anther culture of Chinese radish varieties; ② observe the development of embryos from anther culture; ③ study the response of different genotypes to embryogenesis in anther culture; ④ observe the morphology of regenerated plantlets; ⑤ analyze the ploidy level of regenerated plantlets arising from the anther culture process. [Method]Anthers of 15 genotypes with diverse genetic backgrounds of Chinese radish have been cultured in vitro and induced to undergo embryogenesis and plant formation. [Result] Of 15 genotypes evaluated,four produced embryos. The genotype was the main factor to influence the embryogenesis. The morphology and the ploidy of the regenerated plantlets were observed,and the anther-derived plantlets included a mix of haploids,diploids and hexaploids. Of the plants that regenerated from anther embryos 60% were diploid. [Conclusion] The plantlets had the high ability to double spontaneously.