手机知网 App
24小时专家级知识服务
打 开
生物学
Foundation of protein preparation method and 2-DE analyses of anti-radiation proteins in Antarctic ice microalga Chlamydomonas sp.
<正>Protein preparation is the key step of two - dimensional gel electrophoresis. Antarctic ice microalga Chlamydomonas sp. was used as material in our experiments, from which protein extract methods were compared, and the results showed that improved Trichloroacetic acid (TCA) -acetone fractional precipitation method was one of the fittest methods to protein extraction from Antarctic ice algae. At the same time, we founded a complete 2 - D electrophoresis system fit to Antarctic ice alga proteins. We also analyzed the anti - radiation proteins in Chlamydomonas sp. by 2 - D gel electrophoresis, and two new peptides (21 and 23 kD) were found after exposing to UV radiation.
0 8
手机阅读本文
下载APP 手机查看本文
中国海洋学会海洋生物工程2005年学术年会论文集
2005年
相似文献
图书推荐
相关工具书

搜 索