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丙型肝炎病毒中国株非结构蛋白2~3部分基因cDNA克隆序列分析及表达

范涛;高建恩;陶其敏

  Complementary DNA of partial HCV NS2NS3 protein gene which encodes viral Zn2+dependent metalloprotease and serine protease was amplified by RT nestedPCR from serum of a Chinese patient with chronic hepatitis C.The product was digested withEcoR Ⅰ/XbaⅠ and cloned into pcDNA3.The nucleotide sequence was determined.Comparison of NS2NS3 with the reported typical isolates HCV1,HCVJ,HCC2,HCVJ6,HCVJ8 showed as 73.72%,90.20%,91.02%,64.56%,63.37% identity in nucleotide sequence and 83.24%,92.09%,93.13%,70.88%,67.86% identity in amino acid sequence respectively, so the isolate could be classified as HCV genotype Ⅱ. This gene fragment was cloned into pBV220 to construct recombinant expression vector pBV220NS23.It was expressed efficiently inE.coli. The cloning and expression of HCV NS2NS3 may contribute to the investigation of the relation between structure and function of viral encoded protease.……   
[关键词]:丙型肝炎病毒;非结构蛋白;序列测定;基因表达
[文献类型]:期刊
[文献出处]: 《中国病毒学2000年04期
[格式]:PDF原版; EPUB自适应版(需下载客户端)