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甾体1,4-脱氢和11α-羟基化反应的两种不同微生物转化

徐诗伟;徐清;法幼华

  Two kinds of microorganism,Arthrobacter sp.AX86(1,4dehdrogenator)andAbsidia sp.A28(11αhydroxylator)were used in this experiment.Two different fermentation techniques were performed to accomplish the multiple conversional reactions for producing 16βmethyl11α,17α,21trihydroxy1,4pregnadiene3,20dione(Ⅲ)from 16βmethyl3β,17α,21trihydroxy5αpregnane20one21acetate(I):1)To produce product(Ⅲ)by means of a twostep fermentation method which were independently performed first byArthrobacterand next byAbsiaia,and 2)the product was obtained by a sequential fermentation system of aforesaid two microorganisms in a single fermentor without isolation of the intermediates from the mixture.Our results showed that in both fermentation systems high yield of product was obtained.However,according to the technical simplicity,shorter duration of fermentation cycle and efficient yield of product,the second method is better than the first one.……   
[关键词]:节杆菌;犁头霉;多步转化反应;16β-甲基-11α;17α;21-三羟基孕甾-1;4-二烯-3;20-二酮
[文献类型]:期刊
[文献出处]: 《生物工程学报2000年05期
[格式]:PDF原版; EPUB自适应版(需下载客户端)
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