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SUBCELLULAR SITE OF ANTISPERMATOGENIC EFFECT OF GOSSYPOL AND ITS POSSIBLE MOLECULAR MECHANISM OF ACTION

薛社普;梁德才;费仁仁;陈啸梅;叶世隽;刘裕;吴嬿婉;游美梅;郭秀云

   This paper reports the results of gossypol effects on the testicular mitochondrial ultrastructures and functions. A hypothesis of mechanism of gossypol action based on our experimental data was postulated.Electron microscopic observations confirmed further our previous studies that the mitoehondria of the target germ cells were the most sensitive and the most severely damaged among cellular organelles in response to gossypol. The damages included the swelling, vacuolation, crista depletion, lysis, granular accumulation in matrix and the process of intact mitochondria disintegration. The activity of the mitochondrial marker enzyme, the LDH-X of human spermatozoa, was markedly decreased or suppressed completely after gossypol treatment.The functions of isolated testieular mitochondria determined by Warburg manometric and oxygen electrode polarographic methods indicated that gossypol stimulated respiration but inhibited slightly the oxidative phosphorylation at low concentrations (20-40μM) in vitro. With……