手机知网 App
24小时专家级知识服务
打 开
手机知网|搜索

鲤鱼微卫星分子标记的筛选

魏东旺;楼允东;孙效文;沈俊宝

  A partial common carp genomic library was constructed.45 positive clones were isolated from screening about 2000 clones of the genomic library with a (CA)15probe labelled at the 5end with γ32PATP.Sequencing of these clones was performed with automated sequencer,and 22 microsatellites were isolated.17 primers were designed based on unique sequences flanking each motif with the software Primer3.PCR onCyprinus carpio haematopteruswas carried out with these primers,and all gave expected bands.Annealing temperature of these primers was between 50℃ and 53℃.……   
[关键词]:鲤鱼;微卫星;分子标记
[文献类型]:期刊
[文献出处]: 《动物学研究2001年03期
[格式]:PDF原版; EPUB自适应版(需下载客户端)