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中华鳖7种组织SOX基因表达的RT-PCR分析

聂刘旺;单祥年;汪鸣;郭超文;鲁晓萱

  In this paper, RTPCR analysis on SOX gene of seven tissues from the Chinese softshelled turtle(Pelodiscus sinensis)was studied, and SOX gene fragments of expression from the testicle, brain, heart, kidney and spleen were cloned using RTPCR products.The results show that SOX genes has specific expression in the testicle, brain, spleen, cardiac muscle and kidney and isn t expression in muscle, liver and ovary of femel.The results of sequence reveal that the SOX genes of expression in the testicle areTSSOX1、TSSOX4 TSSOX5 and TSSOX9, and those are TSSOX2 and TSSOX4 in the brain, and this is TSSOX4 in the spleen and heart tissue, and those are TSSOX2 and TSSOX3 in the kidney tissue.This suggests that the SOX gene act important role not only on the sex determination, but also on the development of neural system, immunocyte system and the differentiation of male germ cell.……   
[关键词]:中华鳖;RT-PCR;序列分析;基因表达
[文献类型]:期刊
[文献出处]: 《动物学报2001年06期
[格式]:PDF原版; EPUB自适应版(需下载客户端)