手机知网 App
24小时专家级知识服务
打 开
手机知网|搜索

西瓜花叶病毒中国分离株全基因组核苷酸序列测定

张建新;吴云锋;王睿;罗朝鹏

  Watermelon and melon mosaic disease is the important disease in agricultural production,mostly caused by watermelon mosaic virus.In this paper,the complete nucleotide sequence of watermelon mosaic virus Chinese isolate(WMV-Ch)was firstly determined.Excluding the 3 -terminal poly(A)tail,the genome was 10037 nt long,containing 5 -and 3 -terminal untranslated regions and a large open reading frame.The genome encoded a polyprotein of 3217 amino acids with a predicted Mr of 365.8 kD.Based on comparison with the proposed location of cleavage sites of other potyvirus polyproteins,10 mature proteins were predicted.There were 92.5% nucleotide and 96.4% amino acid identities over entire genome between WMV-Ch and France isolate(WMV-Fr),respectively.The nucleotide identity of the coat protein genes among WMV-Ch and WMV from different countries was all less than 95%,and phylogenetic tree revealed that WMV-Ch was most close to Japanese isolate.……   
[关键词]:西瓜花叶病毒;全基因组;序列测定;结构分析
[文献类型]:期刊
[文献出处]: 《病毒学报2007年02期
[格式]:PDF原版; EPUB自适应版(需下载客户端)