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猪传染性胃肠炎病毒n基因的原核表达及重组蛋白的免疫活性分析

孙东波;冯力;刘杰;时洪艳;佟有恩;刘胜旺;童光志

  A pair of primers containing BamHI and XhoI sites was designed to amplify n gene of 1149bp from the positive pMD18-TN plasmid of transmissible gastroenteritis virus n gene by PCR.PCR product was subcloned into multiple cloning sites of pET-30a containing 6 His·Tag.Recombinant plasmid pET-30a of n gene named pET30a-N was transfected into E.coli BL21 and the bacteria was induced with IPTG at 37℃.It was demonstrated by SDS-PAGE that recombinant N protein expressed in E.coli BL21 was soluble protein and its molecular weight was about 52kD.The result of Western blot test showed that immunoactivity of the recombinant N protein was the same to that of the natural N protein.……   
[关键词]:猪传染性胃肠炎病毒;n基因;原核表达;免疫活性分析
[文献类型]:期刊
[文献出处]: 《病毒学报2006年02期
[格式]:PDF原版; EPUB自适应版(需下载客户端)