Potyvirus属成员基因组全序列的简并引物PCR和RACE扩增方法

　　Basedonmulti-alignmentofcompletepolyproteinaminoacidsequencesofgenusPotyvirus,fivedegeneratedprimersweredesignedTheywereSprimer(5′-GGXAAYAAYAGYGGXCAZCC-3′),pNIa(+)(5′-TNYTGGAAMCAYTGGAT-3′),pCI2(+)(5′-GCXACXAAXATXATXGAXAA-3′),pCI1(+)(5′-GTXGGXTCXGGXAAXTCXAC-3′)andpHC(+)(5′-TGYGAYAAYCAZTTXGA-3′)(X=A,T,CorG:Y=TorC;Z=AorG;N=AorT;M=A,T orG)UsingdegeneratedPCRandmodifiedRACEmethods,aprotocolfordeterminationofcompletegenomesequenceofpotyviruseswasestablishedandprovedtobesuccessfulonfivepotyviruses……