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Effect of Different Culture Media on the Proliferation of Avian Influenza Virus H9 Subtypes in MDCK Cells


  [Objective] To screen the best culture media for the proliferation of avian influenza virus (AIV) H9 subtypes in MDCK cells. [Method] The DMEM containing 10% (V/V) newborn calf serum, low-serum containing medium (MEM-MD-611) and serum-free medium (SFE4Mega) were used to culture the MDCK monolayer cells, which were then inoculated with different dilutions of AIV H9 subtypes, and the 3 kinds of media were also used as the maintenance solution to culture the virus. The cytopathic changes were observed at every 24 h, and the HA titers of the culture supernatants were also determined. [Result] After culturing for 72-96 h, the HA titers of the serum-free media were higher than that of low-serum culture media, while the HA titers were higher in the low-serum media than in the serum containing media. [Conclusion] The 3 kinds of media can all used for the proliferation of AIV, but the low-serum culture medium (MEM-MD-611) and serum-free medium (SFE4Mega) are preferred.……   
[关键词]:Culture medium;Avian influenza virus H9 subtype;MDCK cell;Proliferation
[文献类型]:期刊
[文献出处]: 《Animal Husbandry and Feed Science2012年01期
[格式]:PDF原版; EPUB自适应版(需下载客户端)