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Isolation and Culture of Fetal Fibroblasts of Tibetan Yellow Cattle

CHAMBA Yang-zom

  [Objective][Objective] To isolate fetal fibroblasts from Tibetan yellow cattle and thus to provide the ideal biological material for cloning and cellular biology of animals inhabiting in the Tibet Plateau. [Method] The fibroblast cells were isolated from tissues of Tibetan yellow fetus by explant culture method. The subculture, morphology and dynamics were observed. [Result] The fetal fibroblasts of Tibetan yellow cattle had typical morphology of fibroblast cells. The isolated cells had three growth stages including retention period (0-2 d), logarithmic phase (2-7 d) and platform phase (7-8 d). Its doubling time was 60 h. [Conclusion] Fetal fibroblasts of Tibetan yellow cattle have been successfully cultured.……   
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