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  A cDNA library was constructed from whole silkworm pupae (Bombyx mori) and 1500 clones were randomly selected for sequencing to reveal the gene expression profile of the pupae phase. The general cDNA length is around 100-4000bp, most abundant in 400-1500bp. We have successfully generated high-quality sequence for 1121 clones, which contained 1003 unigenes after being assembled. We compared cDNA sequences to the silkworm {Bombyx mori) genome in GenBank database and the silkworm cDNA database including silkbase and KAIKOcDNA database, and found out 352 novel ESTs and 57 unknown ESTs. After being compared with all available ORF-complete mRNA sequences from the same organism (fruitfly or mosquito) in the RefSeq collection, 385 full-length cDNA were identified. In addition, we analyzed the structure of silkworm mRNA and characterized that 86.2% of silkworm mRNA tailed with poly(A) contained the highly conserved AAUAAA signal and the signal located 10-17 nt upsteam of the putative poly(A). We deduce that AAUAAA box is not only the signal for polyadenylation, but maybe also for transcription stop of pre-mRNA.……   
[关键词]:Bombyx mori;cDNA;construction;ORF;full-length
[文献类型]:会议论文
[文献出处]: 《浙江省生物化学与分子生物学学术交流会论文集2005年
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