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  Human lactoferrin ( hLf ) is a multifunctional iron-binding glycoprotin. In this study, we amplified hLf cDNA by RT-PCR from normal human mammary gland. The N-terminal sequence of the hLf was deduced as GRRRRSV which was identical to the known hLf. We constructed a recombinant virus vBm-hLf harboring hLf gene and exploited the BmN cells as host to produce recombinant human lactoferrin (rhLf ). It was found that a -78 kDa recombinant protein was expressed. Around 13.5 mg/L of rhLf was purified from BmN cells culture medium infected by vBm-hLf and the rhLf was proved to be biologically active. This method established in our study will pave the way for efficient production of rhLf for further utilization of this protein in the future.……   
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