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  Introduction MtDNA was used widely as molecular genetic marker because of its especial structural character, and CO I gene was used far-ranging in the studies of taxonomy,Species identification,genetic diversity of population and molecular evolutionary in vertebrata and invertebrate.In this study,the part sequence of CO I gene was used as molecular marker to identify thirteen indigenous chicken breeds and two import breeds,in order to analysis genetic polymorphism and the connection of molecular biology geography evolvement of interpopulation and intrapopulation about fifteen chicken breeds.……