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Effects of different levels of copper on broiler hepatocyte mitochondrial permeability transition pore

SU R S;WANG R M;CAO H B;PAN J Q;LI C M;SHI D Y;TANG Z X (College of Veterinary Medicine;South China Agricultural University;Guangzhou 510642;China)

  In this work,the effect of different concentrations of copper from different sources on the opening of the mitochondrial non-specific pore was examined.360 one-day-old healthy AA broilers were randomly assigned to twelve experimental treatments.Each treatment was fed a diet contain different concentrations of copper as the form of copper sulfate,copper methionine and tribasic copper chloride(TBCC) for 60 days.The analyzed copper concentrations in the diets(dry matter,DM) respectively were:Ⅹ-Ⅰ=11 mg/kg(the control group),Ⅹ-Ⅱ=110 mg/kg,Ⅹ-Ⅲ=220 mg/kg,andⅩ-Ⅳ=330 mg/kg,Ⅹrepresented copper sulfate(S),copper methionine(M) and tribasic copper chloride(T).On day 12,24,36,48 and 60,livers from different groups were removed for analysis of mitochondrial permeability transition pore(MPTP) and liver copper concentration.The results showed that,under the same copper concentration circumstances,the MPTP of broilers fed copper methionine was the largest,the next one was TBCC,followed by copper sulfate.The MPTP gradually expanded(P<0.05) along with the lengthe ning of the raising day and the increasing of dietary copper concentration.In vitro,we examined the effect of the sucrose-based buffer and KCl plus sucrose-based buffer on MPTP,contained 5μtM Cu~(2+) and 10μM Cu~(2+) respectively.Only 10μtM Cu~(2+) was able to cause permeability transition in the sucrose-based buffer,but 5μtM and 10μM of this cation brought about pore opening obviously in KCl plus sucrose-based buffer.Results of our study indicated that liver copper concentration directly influenced hepatocellular MPTP in vivo,which was relevant to dietary copper source,copper dose and raising period;the effect of Cu~(2+) on mitochondria in vitro was not only relevant to Cu~(2+) concentration,but also relevant to medium.……