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  As a multifunctional cytokine, the transforming growth factor β1 (TGF-β1) was detected in the utero-placental interface during early pregnancy in the pig, and believed to enhance trophoblast attachment to the endometrium. In this experiment, we selected TGF-β1 as the candidate gene affecting litter size in pigs. We detected the polymorphisms of this gene by PCR-SSCP (single-strand conformation polymorphism ) in Large white sows (n=567), four polymorphisms loci were found : C→T mutation at 33nt (GenBank NO: AF461809) in the intron 4, G→A mutation at 179nt (GenBank NO: AJ621785 )in the intron 6, C→T mutation at 1043nt (GenBank NO: AJ621785) in the intron 6, GG→AA linkage mutations at 2490nt and 2494nt respectively (GenBank NO: M23703)found in the 3flanking region. We haplotyped these SNPs into two haplotypes: CGCAA (denote as P) and TATGG (denote as K). The effects of three haplotypic combinations of PP, PK and KK on litter sizes of first parity and second parity were estimated by Linear model, we found that for the first parity litters, the difference between KK and PK was 1.02 pigs per litter (P<0.05) for TNB, 0.67 pigs per litter but not significant (P>0. 1) for NBA, there were 0.49 pigs per litter and 0.37 pigs per litter but not significant difference between KK and PP for TNB and NBA respectively, a slightly difference of 0.53 pigs per litter between PP and PK were existed (0.05<P<0.1) for TNB, but not for NBA.For second parity litters, significant difference between haplotypic combinations for both TNB and NBA were not found (P>0.1) .……   
[关键词]:TGF-β1 gene;litter size;SNP;haplotypic combination
[文献类型]:会议论文
[文献出处]: 《第十次全国畜禽遗传标记研讨会论文集2006年