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  Microsatellite enrichment from AFLP fragments by magnetic beads was introduced in this research. This method can obviate the tedious work of library construction which not only save time but also expense. The genomic DNA was converted into Pre-amplified AFLP fragments by using a few restriction enzymes combination and hybridized with biotin-labeled SSR probes. Then the hybrid mixture was used to incubated with magnetic beads coated with streptavidin. After washing to remove the non-SSR fragments, the eluted single-strand DNA which was cloned and sequenced was largely enriched for microsatellites. Primers can then be designed according to the sequence flanking the repeat motifs and used for polymorphism analysis. Fifty one primers had been developed from six Chinese indigenous goose breeds successfully. Thirty one of them were middle polymorphic better specific and did not link each other. The mean Polymorphism Information Content (PIC) of 6 goose breeds was 0.394-0.448. The highest was in the Shitou (0.448), and in the Yan was the lowest (0.394). The results showed that the 31 microsatellites had middle PIC. These 31 microsatellites could be used in the analysis of genetic diversity, construction of saturated maps, and in some cases, in marker-assisted selection. The whole experiment can be employed as a reliable option for any molecular laboratory to develop SSR markers.……   
[关键词]:microsatellites;AFLP;magnetic beads;goose.
[文献类型]:会议论文
[文献出处]: 《第十次全国畜禽遗传标记研讨会论文集2006年