手机知网 App
24小时专家级知识服务
打 开
手机知网|搜索

  A total of 720 individuals of 12 indigenous chicken populations, geographically localized in South China were genotyped for 30 microsatellite markers to evaluate the genetic variation and genetic distance between populations. The all microsatellites were found to be polymorphic. Heterozygosity and Wright s F-statistics (FIS, FST and FIT) were calculated to determine the genetic variation. Mean values of FIS, FST and FIT per locus were -.0484, 0.083 and -0.305, respectively. Of the 30 microsatellite loci, number of alleles per locus (Na) and effective number of alleles per locus (Ne) ranged from 4 to 11 and 2.157 to 8.019, respectively. The average expected heterozygosity (HE) was 0.669, while the average observed Heterozygosity (HO) was 0.967.The polymorphism information content (PIC) have values between 0.560 and 0.641. Using Nei s standard distance, genetic distance (DA) calculated ranged between 0.088 (Guanxi Sanhuang vs. Nandan Yao) and 0.495 (Huiyang Beard vs. Zhangzhou Game). The topology of phylogenetic trees constructed showed general patterns of relationship and genetic differentiation among the indigenous populations studied. The results provided evidence of the applicability of microsatellite to determining the genetic relatedness among different Chinese indigenous chicken populations and evaluating genetic variations.……