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  Alleles of physiological candidate genes for reproductive traits, insulin-like growth factor-1 (IGF-1) and growth hormone receptor (GHR) were assessed to determine the association with the total egg production (NE), average days of continual egg-laying (ADCE) and number of double-yolked eggs (DYE) in Chinese indigenous breed chicken (Wenchang). PCR-RFLP method was used for genotypes identification. The frequency of restriction enzyme C1/ C2 alleles in the population was 0.53 (C1) and 0.47 (C2) for IGF-1. For GHR-Intron 2 was 0.06 and 0.94 for (A1) and (A2), respectively, while GHR-Intron5 was 0.20 (B1) and 0.80 (B2). IGF-1 polymorphism has an additive effect on egg production trait. The IGF-1 PstI C2C2 genotype had greater number eggs (300d) at 89.03 compared to 82.61 for PstI C1C1 (P<0,05) and (400d) at 137.84 compared to 127.82 for PstI C1C1 (P<0.05). The IGF-1 C2C2 genotype had longer ADCE at 3.38 compared to 2.96 and 2.78 for PstI C1C1 and C1C2 , respectively (P<0.05). GHR polymorphism has additive effect on number of double-yolked eggs (DYE), The GHR HindⅢ A1A1 genotype had greater DYE at 1.00 compared to 0.30 for HindⅢ A2A2 (P<0.05). The current research supports the effects of GHR and IGF-1 genes on reproductive traits of chickens.……   
[关键词]:Chicken;IGF-1;GHR;Reproduction;SNP
[文献类型]:会议论文
[文献出处]: 《第十次全国畜禽遗传标记研讨会论文集2006年