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Transcriptome Profile of Trichoderma afroharzianum LTR-2 Induced by Oxalic Acid

Wu Xiaoqing;Ren He;Lv Yuping;Zhou Hongzi;Zhao Xiaoyan;Zhang Guangzhi;Zhang Xinjian

  Oxalic acid(OA) has been proved to be a pathogenic factor of grey mold fungus Botrytis cinerea,which causes pre-and postharvest crop losses worldwide.Trichoderma afroharzianum LTR-2 is an effective bio-control strain against Botrytis cinerea,and able to eliminate OA at the same time.Although biocontrol mechanisms of Trichoderma,such as mycoparasitism,antibiosis and competition are well studied,the biocontrol meaning of OA elimination effect is still vague.In this article,we analyzed the transcriptome changes of strain LTR-2 induced by 0 and 20 mmol/L OA using RNA-seq technology,each treatment set 3 biological replicates.Totally 653 up-regulated genes and 402 down-regulated genes were found among differentially expressed genes(DEGs),of which the gene functions were mainly related to metabolic process and catalytic activity according to GO terms.Based on pathway enrichment analysis,the DEGs were primarily involved in metabolic pathways,biosynthesis of secondary metabolites and biosynthesis of antibiotics.We selected 4 genes of interest and found oxalate decarboxylase(OXDC) gene(g2863),chitinase gene(g7837),glucanase gene(g5151) and protease gene(g5140) were all significantly up-regulated,and the results were confirmed by RT-PCR analysis.The transcriptome analyses preliminarily showed that OXDC gene was involved in OA elimination,and OA might be a signal which could regulate mycoparasitism in Trichoderma.……   
[关键词]:Trichoderma;Oxalic acid;RNA-seq;Bio-control
[文献类型]:会议论文
[文献出处]: 《中国植物病理学会2017年学术年会论文集2017年