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Genome Sequence of an isolate of Fusarium oxysporum f.sp.melongenae,the Causal Agent of the Fusarium Wilt of Eggplant

Dong Zhangyong;Hsiang Tom;Luo Mei;Xiang Meimei

  Fusarium wilt of eggplant caused by Fusarium oxysporum f.sp.melongenae(Fomg) is an economically important soil-borne disease limiting eggplant production worldwide.This pathogen was initially reported in Japan in 1958 and the first reportin China was in 2005.Here,we report a draft assembly for Fomg isolate 14004,a field strain collected in Guangdong Province,China,where Fusarium wilt of eggplant was observed over ten years ago and is currently endemic.Onemicro gram of genomic DNA was sent for sequencing in Genome Quebec(Montreal,Canada)specifying 100 bp paired-end reads with 300 bp insert.Over 43 million paired-end reads totaling 10.7 Gb were received.The genome was assembled using the programs Velvet v.1.2.10,ABySS v.2.0.1 and SOAPdenovo v.2.04 with odd-numbered kmers between 21 and 91.Assembly quality was assessed by examining the N50 value and by examining the total number of scaffolds produced by the programs.The initial assembly was 54 488 475 bp in length with an N50 value of 568 281 bp,resulting from 4 617 scaffolds.After removing scaffolds that were smaller than 200 bp by using a PERL script,the final assembly consisted of 1 631 scaffolds with a genome size of 53 986 354 bp(G + C content,46.4%).A total of16 485 protein-coding genes were predicted from the assembly with the highest N50(kmer = 77,ABySS) using AUGUSTUS v.3.2.2 based on gene models from Fusarium oxysporum.……