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Screening and Analysis of Effector Proteins from Puccinia triticina

Li Jianyuan;Wei Jie;An Zhe;Zhang Yue;Yang Wenxiang;Liu Daqun

  Leaf rust caused by the fungus Puccinia triticina(Pt) is a major constraint to wheat production worldwide.The molecular events that underlie Pt pathogenicity are largely unknown.Like all rusts,Pt forms a specialized infection structure called haustoria to take nutrients and water from host plant tissue,and to secrete pathogenicity factors called effector proteins.In this study,we extracted the total RNA of urediniospores,germinated urediniospores and infected wheat leaves harvest at 6 d from three different virulence strains of Pt and used Illumina Hiseq sequencing platforms to assemble the transcriptomes and then we got 46008 Unigenes.Candidate effectors were screened through Signal P 4.1,Target P 1.1,TMHMM 2.0 and Effector P,and 325 genes which constitute candidate effectors were identified.qRT-PCR analysis confirmed the expression patterns of 96 candidate effectors and 42 genes showed aspecial increase in various stages which may be involved in the pathogenic process.33 candidate effectors were cloned successfully and 17 were identified to be able to suppress the cell death caused by BAX in tobacco cells.This analysis of candidate effectors is an initial step towards functional research and established the foundation forclarify the pathogenic mechanism of leaf rust pathogens.……   
[关键词]:Puccinia triticina;Effector proteins;Screening
[文献类型]:会议论文
[文献出处]: 《中国植物病理学会2017年学术年会论文集2017年