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Developmentally regulated plasma membrane protein of Nicotiana benthamiana contributes to potyvirus movement and transports to plasmodesmata via the early secretory pathway and the actomyosin system

Chao Geng;Qianqian Cong;Xiangdong Li;Anli Mou;Rui Gao;Jinliang Liu;Yanping Tian

  Intercellular movement of plant viruses requires both viral and host proteins.Previous studies have demonstrated that the P3N-PIPO and cylindrical inclusion(CI)proteins were required for potyvirus cell-to-cell movement.Here,we provided genetic evidence showing that Tobacco vein banding mosaic virus(TVBMV;genus Potyvirus) mutant carrying a truncated PIPO domain of 58 aa residues could move between cells and induce systemic infection in N.benthamiana plants;mutants carrying a PIPO domain of 7,20 or 43 aa residues failed to move between cells and cause systemic infection in this host plant.Interestingly,the movement-defective mutants produced progeny that eliminated the previously introduced stop codons and thus restored their systemic movement ability.We also presented evidences showing that a developmentally regulated plasma membrane protein of Nicotiana benthamiana(referred to as NbDREPP) interacted with both the P3N-PIPO and CI of the movement-competent TVBMV.The knockdown of NbDREPP gene expression in N.benthamiana impeded the cell-to-cell movement of TVBMV.NbDREPP was shown to co-localize with TVBMV P3N-PIPO and CI at plasmodesmata(PD) and traffick to PD via the early secretory pathway and the actomyosin motility system.We also showed that myosin XI-2 is specially required for transporting NbDREPP to PD.In conclusion,NbDREPP is a key host protein within the early secretory pathway and the actomyosin motility system that interacts with two movement proteins and influences virus movement.……