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Overexpression of OsPGIP1 enhances rice resistance to sheath blight

Xijun Chen;Yu Chen;Bin Xu;Linan Zhang;Zongxiang Chen;Yunhui Tong;Shimin Zuo;Jingyou Xu

  Rice sheath blight(SB),caused by necrotrophic pathogen Rhizoctonia solani,is one of the most destructive rice diseases and no major resistance genes are available.Polygalacturonase-inhibiting proteins(PGIPs) are extracellular leucine-rich repeat(LRR) proteins and play important roles in plant defense against different pathogenic fungi by counteracting secreted fungal PGs.However,the role of PGIPs in conferring resistance to rice SB remains to be thoroughly investigated.Here we showed that OsPGIPl is capable of inhibiting PGs derived from R.solani.Our real-time RTPCR results indicated that resistant rice cultivars YSBR1 and Jasmine 85 express significantly higher levels of OsPGIPl than susceptible cultivar Lemont.Our results also show that OsPGIPl is most highly expressed at the late tillering stage in the sheath of YSBR1,coinciding with the critical stage of SB development in field.More importantly,the OsPGIPl level is highly elevated by inoculation with R.solani in resistant cultivars,but not in susceptible Lemont.Overexpression of OsPGIPl significantly increased rice resistance to SB and inhibited tissue degradation caused by R.solani secreted PGs.Furthermore,OsPGIPl overexpression did not affect rice agronomic traits or yield components.Together,our results not only demonstrate the important role of OsPGIPl in combatting the rice SB disease,but also provide a new avenue to the improvement of rice SB resistance by manipulating an endogenous gene.……