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hph gene inserting into the geome of Fusarium effects the biosynthesis of the DON

Cai Yiqiang;Zheng Zhitian;Liu Xiumei;Li Bin;Zhou Mingguo

  With the development of molecular biology,transgenic technology is increasingly widespread application in agriculture.People integrate foreign genes into the genome of crops in order to obtain excellent heritable traits.They pay more attention to the safety of the foreign genes expression in host cells for humans,but the influence of cell metabolism of the host is less research when the foreign genes are inserted.In this study,we choosed Hygromycin B phosphotransferase gene(hph)which was widely used in molecular biology as the foreign gene inserting into the genome of Fusarium graminearum that it could produce secondary metabolites of DON toxin.Then we detected the Tri5 gene expression which had a positive exponential relationship with DON production ability by real-time quantitative PCR(RQ-PCR) to evaluate the DON production ability of the transformations,we found that 28%of the200 transformations we got were down-regulate expressed more than two times and31%were up-regulate expressed more than two times.Therefore,although the foreign gene itself expression was not harmful to humans,it would trigger the metabolic activity of the host strains,such as secretion of harmful metabolic substances which affected human health.……