手机知网 App
24小时专家级知识服务
打 开
手机知网|搜索

   In a single step photolithography, multi-level microfluidic device is fabricated by printing novel architectures on a film photomasks. The whole fabrication process is executed by classical PCB technology without the need to access clean room facilities. Different levels of protruding features on PCB master are produced by exposing a photomask with specifically arrangedwindows and rimsarchitectures, followed by chemical wet etching. Poly(dimethylsiloxane) (PDMS) is then molded against the positive relief master to generate microfluidic device featured with multi-level sandbag structure and peripheral microchannels. This sandbag structure is an analog to traditional dam or weir for particle entrapment. The microstructure does not collapse when subjected to applied pressure, which is suitable for operation on elastic PDMS substrate. Typical immunocytochemcial staining assays were performed in the microdevice to demonstrate the applicability of the sandbag structure for cellular analysis. This simplified microfabrication process employs low-cost materials and minimal specialized equipment and can reproducibly produce mask lines with about 20 μm in width, which is sufficient for most microfluidic applications.……   
[关键词]:Microfluidic device;PDMS;Cell-based assays
[文献类型]:会议论文
[文献出处]: 《第二届全国微全分析系统学术会议论文摘要集2004年