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  Biomineralization is the process in which inorganic ions accumulate in an organized manner in the organic matrices of living organisms. Nacre biomineralization is one of the most typical biomineralization processes, in which the organic matrices in the nacre direct aragonite deposition at different development stages and strictly control the crystal nucleation, orientation and growth dynamics. So far, these organic matrices that have been identified are mainly secreted by the outer epithelia of the mantle edge. To elucidate the mechanism of nacre biomineralization, Pinctada fucata from the South China Sea was used. 768 clones in the outer mantle of P. fucata were obtained by suppression subtractive hybridization (SSH). Reverse Northern dot blotting confirmed that 647 ESTs are highly expressed in the outer mantle of P. fucata . Among them there are 209 ESTs that are expressed 4 times higher in the mantle than in other tissues, and 69 of these ESTs exceed 10 times. Sequencing and match analogy proved that 50 cDNA segments are new ESTs among the 55 submitted ESTs. These differentially expressed cDNA segments that have been identified may have a close relationship with biomineralization processes. Multi - tissue RT - PCR was performed in order to prove that they are indeed highly expressed in mantle tissue. Among them five clones are indeed highly expressed in mantle tissue. Subsequently, we constructed a mantle tissue cDNA library with a pool size exceeding 1.0×106. Using this cDNA library, ten full length novel genes have been obtained through nested PCR. Then we performed mantle in situ hybridization to locate the specific position where these genes are expressed. This is a new way for the bulk cloning of new genes related to biomineralization and may accelerate research on the formation of pearls and treatment of osteoporosis.……   
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