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Optimization of the Fermentation Medium for Alpha-Galactosidase Production from Aspergillus foetidus ZU-G1 Using Response Surface Methodology

Caiquin LIU

  The optimization of fermentation medium for alpha-galactosidase production by Aspergillus foetidus ZU-G1 was investigated in shaker flask fermentation.A one-factor-at-a-time experiment was used to screen the preferable nutriment(carbon sources,nitrogen sources,and essential elements) for alpha-galactosidase production. A fractional factorial design was used to screen the main 5 factors,soybean meal,wheat bran,KH_2PO_4,FeSO_4·7H_2O, and the medium initial pH,that affected the production of alpha-galactosidase.The central composite experimental design was further adopted to derive a statistical model for optimizing the composition of the fermentation medium. The experimental results showed that the optimum fermentation medium for alpha-galactosidase production by Aspergillus foetidus ZU-G1 was composed of 3.2%soybean meal(w/v),2%wheat bran(w/v),0.1%KH_2PO_4(w/v), and 0.05%FeSO_4·7H_2O(w/v);initial medium pH was 6.31.The results further predicted that alpha-galactosidase activity reached 64.75 U/mL after 96-h incubation in this medium,which was approximately 7 times higher than that incubated in the nonoptimized medium.The time course of alpha-galactosidase production in the optimized medium composition was also carried out to validate themodel.……