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Antitumor immunity induced by DNA vaccine encoding alpha-fetoprotein/heat shock protein 70


   AIM:To construct a DNA vaccine encoding human alpha-fetoprotein(hAFP)/heat shock protein 70(HSP70),and tostudy its ability to induce specific CTL response and itsprotective effect against AFP-expressing tumor.METHODS:A DNA vaccine was constructed by combininghAFP gene with HSP70 gene.SP2/0 cells were stablytransfected with pBBS212-hAFP and pBBS212-hAFP/HSP70eukaryotic expression vectors.Mice were primed andboosted with DNA vaccine hAFP/HSP70 by intramuscularinjection,whereas plasmid with hAFP or HSP70 was usedas controls.ELISPOT and ELISA were used to detect IFN-γ-producing splenocytes and the level of serum anti-AFPantibody from immunized mice respectively.In vivo tumorchallenge was measured to assess the immune effect ofthe DNA vaccine.RESULTS:By DNA vaccine immunization,the results ofELISPOT and ELISA showed that the number of IFN-γ-producing splenocytes and the level of serum anti-AFPantibody were significantly higher in rhAFP/HSP70 groupthan in hAFP and empty plasmid groups(95.50±10.90IFN-γ spots/10~6 cells vs 23.60±11.80 IFN-γ spots/10~6 cells,7.17±4.24 IFN-γ spots/10~6 cells,P<0.01;126.50±8.22μg/mLvs 51.72±3.40 μg/mL,5.83±3.79 μg/mL,P<0.01).Thetumor volume in rhAFP/HSP70 group was significantly smallerthan that in pBBS212-hAFP and empty plasmid groups(37.41±7.34 mm~3 vs381.13±15.48 mm~3,817.51+16.25 mm~3,P<0.01).CONCLUSION:Sequential immunization with a recombinantDNA vaccine encoding AFP and heat shock protein70 couldgenerate effective AFP-specific T cell responses and inducedefinite antitumor effects on AFP-producing tumors,whichmay be suitable for some clinical testing as a vaccine forHCC.Wang XP,Liu GZ,Song AL,Li HY,Liu Y.Antitumor immunityinduced by DNA vaccine encoding alpha-fetoprotein/heat shockprotein 70.World J Gastroenterol 2004;10(21):3197-3200http://www.wjgnet.com/1007-9327/10/3197.asp……