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Genetic Analysis of Gynogenetic and Common Populations of Verasper moseri Using SSR Markers


  The genetic structure and variation of the artificial meio- gy- nogenetic population and common population of barfin flounder (Verasper moseri) were analyzed using eight microsatellite markers. A total of 29 alleles were detected, of which 23 alleles were in the artificial gynogenetic population while 29 alleles were in the control group. The average ob- served heterozygosity (HO) of eight loci in the control group (0.526 8) was several times higher than that (0.185 8) in the gynogenetic population. The results indicate that the genetic diversity of the control group was much higher than that of the gynogenetic population of barfin flounder (Verasper moseri). Most loci significantly deviated from Hardy-Weinberg equilib- rium (HWE) after Bonferroni correction (p <0.005 56) in the gynoge- netic population, while four loci deviated from HWE in the control group. The coefficient of gene differentiation (GST) was 0.131 0, and the genetic distance was 0.171 8 between the two populations, suggesting a signifi- cant genetic differentiation between the two populations.……