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牛肝辅酶Ⅱ依赖性视黄醇脱氢酶cDNA的克隆及组织表达

王桂玲;黄东阳;刘戈飞;杜晶

  NADP(H)dependent retinol dehydrogenase/reductase (NRDR) was an important retinoic acid synthase, which was first purified from rabbit liver in 1997. In order to study the function of the NRDR gene,the fulllength cDNA of bovine NRDR was cloned. According to the conserved sequences of human, mouse and rabbit NRDR cDNA,a pair of primers was designed to amplify a 294 bp DNA fragment of bovine liver NRDR, and thenthe fulllength of NRDR cDNA (AF487454) was cloned by using 3′RACE and 5′RACE. All thecloned NRDR proteins consist of 260 amino acid residues and showed high identity among them. The tripeptide of human, mouse and rabbit NRDR Cend wasSRL and that of bovine NRDR Cend wasSHL, but both were considered to be peroxisomal target signal 1 (PTS1). RTPCRdemonstrated that NRDR gene was expressed in liver, heart, lung, kidney, stomach and intestine, and was not found in pancreas, muscle, artery and skin. The fulllength bovine NRDR cDNA has been successfully cloned and the sequence was analyzed. It provided a reliable foundation to investigate the biological function of this protein.……