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Analysis of Chemical Constituents of Petroleum Ether Extract from Sagina japonica(Sw.) Ohwi and Screening of Its Anti-tumor Activity


  [Objective] To analyze the chemical constituents of petroleum ether extract from Sagina japonica(Sw.) Ohwi and study its anti-tumor activity.[Method] GC-MS technology was applied to analyze and identify the chemical constituents of petroleum ether extract from Sagina japonica(Sw.) Ohwi.MTT method and SRB method were used to determine the anti-tumor activity of the extract on three human cancer cell lines(human leukemia K562 cell line,human cervical carcinoma Hela cell line and human breast carcinoma MCF-7 cell line).[Result]Twenty-five kinds of constituents were isolated and identified from petroleum ether extract of Sagina japonica(Sw.) Ohwi,among which the content of aliphatic acid was the highest and that of linoleic acid was 49.318%.There was weak anti-tumor activity of petroleum ether extract from Sagina japonica(Sw.) Ohwi on K562 cell line,while there was no anti-tumor activity of that on Hela cell line and MCF-7 cell line.[Conclusion] The research would provide basis for the comprehensive application of Sagina japonica(Sw.) Ohwi in clinic.……