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Isolation and Determination of Catechin from Cynomorium songaricum Rupr


  [Objective] To establish the isolation and determination methods of catechin from Cynomorium songaricum Rupr.[Method] The dried Cynomorium songaricum Rupr.powder was extracted with 50% ethanol through ultrasonic and purified with macroporous absorption resin.HPLC conditions were as follows:C18 column(250 mm×4.6 mm,5 μm);mobile phase of acetonitrile-0.2acetic acid(15:85 V/V);room temperature as column temperature;flow rate of 1ml/min,detective wavelength of 280 nm.[Result] Catechin was concentrated in 35% ethanol elution fraction;the sample and the negative control were separated well,the linear range of catechin was 0.005-0.04 mg/ml(r = 0.999 9),the recovery rate was 98%,RSD was 0.658%.[Conclusions] Catechin can be isolated and purified from Cynomorium songaricum Rupr.effectively;HPLC is suitable for the determination with the advantages of small deviation and good reproducibility.……   
[关键词]:Cynomorium songaricum Rupr.;Catechin;Macroporous absorption resin;HPLC;China
[文献类型]:期刊
[文献出处]: 《Medicinal Plant2010年08期
[格式]:PDF原版; EPUB自适应版(需下载客户端)