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HPLC-ELSD Fingerprint of Bulbus Fritillariea unibractea from Different Producing Areas


  [Objective]To establish a HPLC-ELSD fingerprint analysis method of Fritillaria unibractea,and provide references for the quality control of Fritillaria unibracteata.[Methods]The separation was performed on an Agilent Extend-C18(4.6 mm × 250 mm,5 μm)with gradient mobile phase consisting of water(0.02% Triethylamine) and carbinol.The flow rate was 1.0 ml/min.The temperature of drift tube was 90 ℃ and the volume flow of carrier gas was 2.3 L/min.The sampling volume was 10 μl and the temperature of column was 25 ℃.[Results]The fingerprint chromatogram of 16 batches of Fritillaria unibractea from different producing areas was set up,with 15 common peaks shown.[Conclusion]The method was accuracy and can be used in the identification and quality control of Fritillaria unibractea.……   
[关键词]:Fritillaria unibractea;HPLC-ELSD;Fingerprint;China
[文献类型]:期刊
[文献出处]: 《Medicinal Plant2010年08期
[格式]:PDF原版; EPUB自适应版(需下载客户端)