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Optimization of Separation Condition of Triterpenoids from Ganoderma lucidum under HPLC


  [Objective]To optimize the separation condition of triterpenoids from Ganoderma lucidum.[Method]The effects of different extraction solvents,different mobile phases and different sampling volume on the separation of triterpenoids were studied by RP-HPLC,and the triterpenoids of 4 kinds of health care products were determined under the optimum condition.[Result]The optimum condition was as follows:extraction solvents of chloroform,mobile phase of 0.2% acetic acid-water,sampling volume of 10-30 μl,and the chromatogram of 2 kinds of the health care products were similar with that of Ganoderma lucidum medicinal material.[Conclusion]The optimum conditions were obtained,and the 4 kinds of health care products were determined by this method,which could used to control the extraction quality of Ganoderma lucidum and evaluate the health care products.……   
[关键词]:Ganoderma lucidum;triterpenoids;High performance liquid chromatography;China
[文献类型]:期刊
[文献出处]: 《Medicinal Plant2010年08期