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Determination of Resveratrol and the Other Three Constituents of Polygonum Cusprdatum Sieb.et Zucc by HPLC


  [Objective] To establish a method for simultaneous determination of resveratrol,polydatin,emodin and physcion in Giant Knotweed Rhizome by HPLC.[Methods] The HPLC method was established with DIKMA C18 column(200 mm×4.6 mm,5 μm)and a mobile phase of acetonitrile-0.1% phosphoric acid.The flow rate was 1.0 ml/min;the detection wavelength was 303 nm and the column temperature was 30 ℃.[Results]the linear regression equation of resveratrol,polydatin,emodin and physcion were y = 7 116 169x + 15 631(r=0.999 9),y= 395 313x + 50(r=0.999 9),y=2 752 869x-266 4(r=0.999 9) and y = 463 866x-1 413(r=0.999 9),and present good linear relationship within 0.026-0.520 μg,0.045-0.765 μg,0.026-0.520 μg and 0.026-0.442 μg,respectively.[Conclusion]The method is feasible with good repeatability,and can be used for quality control of Giant Knotweed Rhizome.……   
[关键词]:Giant Knotweed Rhizome;Resveratrol;Polydatin;Emodin;Physcion;HPLC;China
[文献类型]:期刊
[文献出处]: 《Medicinal Plant2010年08期