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Comparison of Hypericin Contents in Hypericum hubeiense and Hypericum perforatum


  [Objective]The contents of hypericin in Hypericum hubeiense and Hypericum perforatum were comparatively determined,aiming at providing basis for the development and utilization of Hypericum hubeiense.[Method]HPLC was used,and the chromatographic conditions were as follows:Supelcosil ODS(4.6 mm×250 mm,5 μm) column,mobile phase of methanol-acetonitrile-water-0.3% aqueous solution of phosphoric acid(v:v=45:50:4.5:0.5,adjusting the pH to 6 with triethylamine),flow rate of 1 ml/min and detection wavelength of 590 nm.[Result]The content of hypericin in Hypericum hubeiense was 3 times as that in Hypericum perforatum growing in the same environment and decreased with the increasing elevation of its growth.Meanwhile,the hypericin content in the flowers and young fruits of Hypericum hubeiense was the highest which was 0.617%-0.877%,and that in leaves and ripe fruits was about 0.35%,while that in roots and stems was extremely low.[Conclusion]This will provide theoretical basis for the artificial cultivation,scientific harvest and development of Hypericum hubeiense.……   
[关键词]:Hypericum hubeiense;Hypericum perforatum;Hypericin;Comparing contents;China
[文献类型]:期刊
[文献出处]: 《Medicinal Plant2010年08期
[格式]:PDF原版; EPUB自适应版(需下载客户端)