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AP1基因转化地被菊品种‘玉人面’的研究

吕晋慧;吴月亮;孙磊;张启翔

  The flower s meristematic characteristic gene AP1 was introduced into Chrysanthemum morifolium cv. ‘Yu Ren Mianmediated by Agrobacterium tumefaciens.The factors influencing genetic transformation protocol were studied.The results showed that the leaf explants precultured for 2~8 hours or not precultured were best for transformation by A. tumefaciens. The suitable concentration of bacterial and the time for infecting was OD_ 600 0.5 for 10 minutes. Leaves were cocultivated with bacterial at 23~25 ℃ for 2 days,then delayed selection for 3 days. Kan~r plant were selected by increased selective press from 5 mg·L ~ -1 G418 to 7.5 mg5L~ -1 G418 followed by 10 mg5L~ -1 G418. The integration of AP1 gene into C. morifolium ‘Yu Ren Mianwas confirmed by PCR and Southern blotting.Two of transgenic plants bloomed 15 days earlier than untransformed plants.……   
[关键词]:地被菊;AP1基因;根癌农杆菌;遗传转化
[文献类型]:期刊
[文献出处]: 《林业科学2007年09期