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The effect of lipopolysaccharides on the expression of CD14 and TLR4 in rat Kupffer cells


   OBJECTIVE: To assess the effect of lipopolysaccharides (LPS) on the expression of CD14 and TLR4 inrat Kupffer cells (KCs).METHODS: In rat KCs induced by LPS, the changes of CD14 and TLR4 expression were measured byRT-PCR and immunohistochemistry, and the expressions of TNF-αmRNA, IL-6mRNA or theconcentrations of TNF-α, IL-6 were estimated by in situ hybridization, radioimmunoassay, and others.RESULTS: The expressions of CD14 and TLR4 in KCs induced by LPS were markedly increased in adose-dependent manner (10 mg/L-1μg/L) or in a time-dependent manner (0.5 h-24 h), with thepeaked expression of CD14 at 3-6 hours. The expressions of CD14 and TLR4 in KCs stimulated by theactive mediators from KCs which had been exposed to LPS for 1 hour were obviously increased.CONCLUSIONS: There is a close relationship between LPS or the active mediators from KCs induced byLPS and the expressions of CD14, TLR4. It is implied that the increase of TLR4, CD14 expression maybe induced by LPS within 1--3 hours, and further increase of TLR4, CD14 expression may be correlatedwith the cytokines produced bv KCs.……