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Allolymphocyte apoptosis induced by soluble liver specific antigen


   OBJECTIVE: To investigate the alloimmunogenicity of liver specific antigen and its effects onallolymphocytes.METHODS: Liver specific antigen isolated from inbred F344 rats was used as immunogen toimmunize inbred Lew rats through different immunization pathways such as low-dose long-term hindfootpad, high-dose portal vein and thymus immunization. Western blotting, DNA fragments gelelectrophoresis, mixed lymphocyte culture (MLC) and mixed lymphocyte hepatocyte culture (MLHC)were employed to analyze the immune state after immunization.RESULTS: At the time point of sampling, different degree of specific low immunoresponses appeared inall immunized groups as well as cyclophosphamide (CY) treated group. Compared with group I, othergroups expressed caspase-3 significantly as detected by using Western blotting. DNA fragment gelelectrophoresis of splenocytes showed lymphocyte apoptosis. Compared with the group I, MLC of theexperimental groups showed no significant changes except that of the group V, whereas MLHC decreasedmarkedly (P<0.05).CONCLUSIONS: Liver specific antigen not only has alloimmunogenicity to induce alloimmunoreactionbut induce antigen specific low immunoresponses and antigen specific lymphocyte apoptosis by high-doseor low-dose long-term immunization. It may be an important transplantation antigen that may lead to anovel way to liver transplantation immunotolerance.……