手机知网 App
24小时专家级知识服务
打 开
手机知网|搜索

Inhibition of Ampicillin-resistance in Bacteria by Modified DNAzymes


  To overcome ampicillin-resistance of bacteria which is believed to attribute their endogenous β-lactamase,we designed three 10-23 DNAzymes(Dz1,Dz2,Dz3) targeting the coding region of β-lactamase mRNA and examined their inhibitory capabilities of the ampicillin-resistance of TEM-1 and TEM-3 bacteria. Dz1 was a traditional 10-23 DNAzyme,Dz2 was the mutant of Dz1 by addition of the protected nucleotide to each arm of the enzyme,and Dz3 was a mutant of Dz1 at antisense arms of which phosphorothioate modifications were made. Kinetic analysis,bacterial growth,and β-lactamase activity measurement showed that all the three DNAzymes worked efficiently in vitro and in vivo. A 9 hours bacterial growth inhibition test showed that the inhibition rates of TEM-1 bacteria by Dz1,Dz2,and Dz3 were 27%,50%,and 29%,respectively. In addition,the inhibition rates of TEM-3 bacteria by those three DNAzymes were found to be 49%,58%,and 45%,respectively. The current findings suggest that DNAzymes may become potential candidates of alternative inhibitors for bacteria drug-resistance.……   
[关键词]:DNAzyme;Drug-resistance;β-Lactamase;Ampicillin;Antisense drug
[文献类型]:期刊
[文献出处]: 《Chemical Research in Chinese Universities2008年04期
[格式]:PDF原版; EPUB自适应版(需下载客户端)