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Phenotypic and Functional Analysis of LCMV gp33-41-Specific CD8 T Cells Elicited by Multiple Peptide Immunization in Mice Revealed the Up-regulation of PD-1 Expression on Antigen-Specific CD8 T Cells


  The phenotype and function of antigen-specific CD8 T cells are closely associated with the efficacy of a therapeutic vaccination.Here we showed that multiple immunizations with LCMV gp33-41 peptide(KAV)in Freund s adjuvant could induce KAV-specific CD8 T cells with low expression of CD127 and CD62L molecules.The inhibitory receptor PD-1 was also expressed on a substantial part of KAV-specific CD8 T cells,and its expression level on KAV-specific CD8 T cells in spleen and lymph nodes was much higher when compared to those in peripheral blood.Furthermore,KAV-specific CD8 T cells could specifically kill KAV-pulsed target cells in vivo but the efficiency was low.These data suggest that prime-boost vaccination schedule with peptide in Freund s adjuvant can elicit antigen-specific CD8 T cells of effector-like phenotype with partial functional exhaustion,which may only provide short-term protection against the pathogen.……   
[关键词]:LCMV;CD8 T cell;peptide vaccine;phenotype;PD-1
[文献类型]:期刊
[文献出处]: 《Cellular Molecular Immunology2007年06期